Anti-UmuD | Protein UmuD
SKU: BTL-AGR-A-02323 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS21 4546 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 100 µl |
| Description | The products of umuD , umuC , and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase. |
| Applications | Western blot (WB) |
| Immunogen | Highly purified, full length, recombinant UmuD protein from E.coli, UniProt: P0AG11 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Liquid |
| Molecular Weight | 17 kDa (intact) 14 kDa (processed) |
| Dilution Range | 1 : 3000 (WB) |
| Uniprot Number | P0AG11 |
| Purity | Serum. Contains 0.05 % sodium azide. |
| Specie Reactivity | Escherichia coli |
| Storage | Store at -20°C and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |