RuvA (protein, positive control)
SKU: BTL-AGR-A-02318 | Brand: Agrisera
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| Cat Number | AS21 4543P |
|---|---|
| Category | Primary Antibody |
| Pack Size | 20 µg |
| Description | RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between. |
| Applications | Western blot (WB) |
| Form | Liquid |
| Molecular Weight | 22 kDa (monomer) |
| Uniprot Number | P0A809 |
| Purity | Contains 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 90 % pure by SDS-PAGE. |
| Storage | Store at 4°C or -20°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |