Home / Antibody / Anti-BFP, GFP, YFP | Fluorescent Protein, clone 3A6 , 100 µg
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Anti-BFP, GFP, YFP | Fluorescent Protein, clone 3A6

SKU: BTL-AGR-A-02279 | Host: Mouse | Brand: Agrisera

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Cat Number AS20 4485
Category Primary Antibody
Pack Size 100 µg
Description Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Applications ELISA (ELISA), Western blot (WB)
Immunogen Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Clonality Monoclonal
Host Mouse
Form Lyophilized
Dilution Range 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)
Purity Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Specie Reactivity BFP, GFP, YFP
Reconstitution Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Storage Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Note The product is for research use only