Anti-BirA (mutated/TurboID)
SKU: BTL-AGR-A-02226 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS20 4440 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 50 µg |
| Description | Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. |
| Applications | Immunofluorescence (IF), Western blot (WB) |
| Immunogen | Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Lyophilized |
| Molecular Weight | 35 kDa |
| Dilution Range | 1 : 5000 (WB) |
| Purity | Immunogen affinity purified serum in PBS pH 7.4. |
| Specie Reactivity | BirA (mutated/TurboID) |
| Expected Reactivity | mini TurboID |
| Reconstitution | For reconstitution add 50 µl, of sterile water |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |