Anti-12S seed storage protein CRC
SKU: BTL-AGR-A-02189 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS20 4403 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 200 µg |
| Description | Major 12S seed storage protein CRC (globulin) is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond. Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA). 12S CRC protein is cleaved to alpha and beta chains. Cellular localisation: vacuole.Alternative protein names: Cruciferin 3, Cruciferin C Legumin-type globulin Cruciferin1, Legumin-type globulin storage protein CRU1. |
| Applications | ELISA (ELISA), Immunolocalization (IL) using electron microscopy, Immunohistochemistry (IHC), Western blot (WB) |
| Immunogen | Native, 12S globulin alpaha subunit purified from Arabidopsis thaliana and excised from SDS-PAGE gel. UniProt: Q96318, TAIR: At4g28520 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Liquid at 2 mg/ml. |
| Molecular Weight | 58 | 30 kDa (alpha subunit) |
| Dilution Range | Assay dependent (ELISA), 1: 50 (IL by electron microscopy), 1: 100 (IHC), 1: 3000 - 1: 10 000 (WB) |
| Uniprot Number | Q96318 |
| Purity | Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized. |
| Specie Reactivity | Arabidopsis thaliana |
| Expected Reactivity | Species of your interest not listed? Contact us |
| Storage | Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |