Anti-FITC | fluorescein isothiocyanate, Biotin-conjugated (monoclonal, Clone NI 239)
SKU: BTL-AGR-A-02055 | Host: Mouse | Brand: Agrisera
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| Cat Number | AS18 4214 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 200 µg |
| Description | FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems. |
| Applications | ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB) |
| Immunogen | Highly purified fluorescein isothiocyanate isomer 1, |
| Clonality | Monoclonal |
| Host | Mouse |
| Form | Lyophilized |
| Dilution Range | The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB). |
| Purity | Purified IgG.1 in PBS pH 7.2, biotin conjugated. |
| Specie Reactivity | FITC molecule |
| Reconstitution | For reconstitution add 0.5 ml of sterile water |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C. |
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| Note | The product is for research use only |