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Anti-mCherry (chicken antibody)

SKU: BTL-AGR-A-02023 | Host: Chicken | Brand: Agrisera

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Cat Number AS18 4178
Category Primary Antibody
Pack Size 100 µl
Description mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, however it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Applications Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
Immunogen Recombinant full length mCherry expressed and purified from E. coli.
Clonality Polyclonal
Host Chicken
Form Lyophilized
Dilution Range 1 : 1000 (ICC), 1: 2000 (IF), 1 : 2000-1 : 5000 (WB)
Purity Purified, total IgY (chicken egg yolk immunoglobulin) with 3% trehalose.
Specie Reactivity mCherry
Expected Reactivity mScarlett
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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