Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated
SKU: BTL-AGR-A-00914 | Host: Goat | Brand: Agrisera
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| Cat Number | AS10 852 |
|---|---|
| Category | Secondary Antibody |
| Pack Size | 0.5 mg |
| Description | Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays. |
| Applications | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Immunogen | Purified Rabbit IgG (H&L) |
| Clonality | Polyclonal |
| Host | Goat |
| Form | Lyophilized |
| Dilution Range | The optimal working dilution should be determined by the investigator |
| Purity | Immunogen affinity purified goat IgG, F(ab)'2 fragment. |
| Specie Reactivity | Rabbit IgG (H&L) |
| Expected Reactivity | Rabbit IgG (H&L) |
| Reconstitution | For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
| Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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| Note | The product is for research use only |