CP43' | IsiA homolog of plant CP43 positive control/quantitation standard
SKU: BTL-AGR-A-00507 | Brand: Agrisera
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| Cat Number | AS10 111S |
|---|---|
| Pack Size | 250 µl |
| Description | This product is a recombinant protein standard, source: Synechocystis strain PCC 6803. |
| Applications | Western blot (WB) |
| Form | Lyophilized |
| Molecular Weight | 27 kDa (slightly larger than native protein due to His-tag) |
| Dilution Range | Standard curve: 3 loads are recommended (2.5 and 10 μl).For most applications a sample load of 0.2 μg of chlorophyll will give a IsiA signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing. |
| Reconstitution | For reconstitution add 225 µl of milliQ water |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
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| Note | The product is for research use only |