Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG
SKU: BTL-AGR-A-00428 | Host: Donkey | Brand: Agrisera
₹ 0.00
| Cat Number | AS10 1019 |
|---|---|
| Category | Secondary Antibody |
| Pack Size | 1 mg |
| Description | Donkey anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG, and affinity purified using solid phase rabbit IgG. |
| Clonality | Polyclonal |
| Host | Donkey |
| Form | Lyophilized |
| Dilution Range | The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt) |
| Purity | Immunogen affinity purified IgG. |
| Reconstitution | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
| Storage | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
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| Note | The product is for research use only |