SKU: BTL-AGR-A-00401 | Host: Donkey | Brand: Agrisera
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Cat Number | AS09 631 |
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Category | Secondary Antibody |
Pack Size | 1 mg |
Description | Donkey anti-Sheep IgG (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. |
Immunogen | Purified Sheep IgG, whole molecule |
Clonality | Polyclonal |
Host | Donkey |
Form | Lyophilized |
Dilution Range | 1 : 20-1 : 2000 for most applications |
Purity | Immunogen affinity purified donkey IgG. |
Reconstitution | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
Storage | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
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Note | The product is for research use only |