SKU: BTL-AGR-A-00378 | Host: Goat | Brand: Agrisera
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Cat Number | AS09 603 |
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Category | Primary Antibody |
Pack Size | 1 mg |
Description | Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays. |
Applications | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
Immunogen | Purified chicken IgY, whole molecule |
Clonality | Polyclonal |
Host | Goat |
Form | Lyophilized |
Dilution Range | 1 : 10 000 -1 : 150 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 20 000 and 1 : 10 000 (WB) |
Purity | Immunogen affinity purified goat IgG. |
Specie Reactivity | Chicken IgY heavy and light chains (H&L). |
Reconstitution | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
Storage | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
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Note | The product is for research use only |