Goat anti-Rabbit IgG (H&L), HRP conjugated
SKU: BTL-AGR-A-00376 | Host: Goat | Brand: Agrisera
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| Cat Number | AS09 602 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 1 mg |
| Description | Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays. |
| Applications | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Immunogen | Purified Rabbit IgG, whole molecule, |
| Clonality | Polyclonal |
| Host | Goat |
| Form | Lyophilized |
| Dilution Range | 1 : 50 000 -1 : 90 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1: 10 000 -1 : 50 000 (WB) |
| Purity | Immunogen affinity purified using solid phase rabbit IgG. |
| Specie Reactivity | Based on IEP, this antibody reacts with: rabbit IgG heavy chains and light chains on all rabbit immunoglobulins |
| Reconstitution | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily |
| Storage | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water, add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
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| Note | The product is for research use only |