Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody)
SKU: BTL-AGR-A-00182 | Host: Rabbit | Brand: Agrisera
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| Cat Number | AS07 260 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 50 µg |
| Description | The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves. |
| Applications | Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB) |
| Immunogen | KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Lyophilized |
| Molecular Weight | 90-95 kDa (Arabidopsis thaliana, depending upon an isoform) |
| Dilution Range | 1 : 600-1 : 1000 (IF), 1 : 100 (IL), 1 : 1000-1 : 10 000 (WB) |
| Uniprot Number | P20649 , P19456 , P20431 , Q9SU58 , Q9SH76 , Q9LY32 , Q9M2A0 , Q9M2A0 , Q42556 , Q9LV11 , Q9FNS3 |
| Purity | Immunogen affinity purified serum in PBS pH 7.4. |
| Specie Reactivity | Actinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Salicornia bigelovii, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Vicia faba, Zea mays |
| Expected Reactivity | Amaranthus hypochondriacus,Avena sativa,Beta vulgaris, Cyanidioschyzon merolae, Dunaliella spp., Galdieria sulphuraria, Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Prosopis alba, Rosa chinensis Jacq., Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis Species of your interest not listed? Contact us |
| Reconstitution | For reconstitution add 50 µl of sterile water |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C. |
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| Note | The product is for research use only |