Anti-RFA | Baker's yeast replication factor A
SKU: BTL-AGR-A-00153 | Host: Rabbit | Brand: Agrisera
₹ 0.00
| Cat Number | AS07 214 |
|---|---|
| Category | Primary Antibody |
| Pack Size | 50 µl |
| Description | Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody |
| Applications | Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Western blot (WB) |
| Immunogen | RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa); overexpressed in E.coli and purified by chromatography; no affinity tags were added to any of three subunits |
| Clonality | Polyclonal |
| Host | Rabbit |
| Form | Lyophilized |
| Molecular Weight | 70 + 30 + 14 kDa |
| Dilution Range | ChIP, 1 : 20 000 (WB) |
| Uniprot Number | P22336 , P26754 |
| Purity | Serum |
| Specie Reactivity | Saccharomyces cerevisiae |
| Expected Reactivity | Saccharomyces cerevisiae |
| Reconstitution | For reconstitution add 50 µl of sterile water |
| Storage | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| View Document | |
| Product Url | View Document |
| Note | The product is for research use only |